miR395 is a general component of the sulfate assimilation regulatory network in Arabidopsis |
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Authors: | Colette A Matthewman Cintia G Kawashima Dalibor Húska Tibor Csorba Tamas Dalmay Stanislav Kopriva |
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Institution: | a John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK b School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK c Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Czech Republic |
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Abstract: | In plants, microRNAs play an important role in many regulatory circuits, including responses to environmental cues such as nutrient limitations. One such microRNA is miR395, which is strongly up-regulated by sulfate deficiency and targets two components of the sulfate uptake and assimilation pathway. Here we show that miR395 levels are affected by treatments with metabolites regulating sulfate assimilation. The precursor of cysteine, O-acetylserine, which accumulates during sulfate deficiency, causes increase in miR395 accumulation. Feeding plants with cysteine, which inhibits sulfate uptake and assimilation, induces miR395 levels while buthionine sulfoximine, an inhibitor of glutathione synthesis, lowers miR395 expression. Thus, miR395 is an integral part of the regulatory network of sulfate assimilation. |
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Keywords: | APS adenosine-5&prime -phosphosulfate ATPS ATP sulfurylase APR adenosine-5&prime -phosphosulfate reductase APK APS kinase PAPS 3&prime -phosphoadenosine-5&prime -phosphosulfate SOT sulfotransferase GFP green fluorescent protein PSK phytosulfokine |
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