Effects of nucleotides and divalent cations on phospholipase activity in Saccharomyces cerevisiae |
| |
Authors: | Wolfgang Witt Peter Hampel Klaus Böcker Angelika Mertsching |
| |
Affiliation: | (1) Institut für Pharmakologie und Toxikologie, Philipps-Universität Marburg, Lahnberge, D-3550 Marburg, Federal Republic of Germany |
| |
Abstract: | Divalent cations activate the lysophospholipase and transacylase reactions catalyzed by the same enzymes in the yeast Saccharomyces cerevisiae. The activation was observed at neutral pH, but not at the pH optimum of lysophospholipase/transacylase, near 3.5. Adenine nucleotides, especially AMP and ADP, are strong inhibitors of the same group of enzymes. Half maximal inhibition by AMP was found at a concentration of about 20 M. The inhibition by nucleotides in low concentrations is enhanced by divalent cations. |
| |
Keywords: | Saccharomyces cerevisiae Yeast Phospholipase B Lysophospholipase Enzyme inhibition AMP Unesterified fatty acids |
本文献已被 SpringerLink 等数据库收录! |