Hafnia alvei lipopolysaccharides: Isolation, sugar composition and SDS-PAGE analysis |
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| Authors: | Anna Romanowska Ewa Katzenellenbogen Ma&#;gorzata Ku&#;akowska rzej Gamian Danuta Witkowska Marian Mulczyk El&#;bieta Romanowska |
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| Institution: | Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wroc?aw, Poland |
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| Abstract: | Abstract Lipopolysaccharides (LPS) of 33 strains of Hafnia alvei were isolated and purified. LPS content of the dry bacterial mass ranged from 1.2 to 4.5%. All examined lipopolysaccharides contained glucose, glucosamine, heptose, 3-deoxy-octulosonic acid and often galactose. Rhamnose, mannose, galactosamine, mannosamine and unidentified amino sugars were found in some H. alvei strains. Sialic acid was present in LPS of one strain. d -3-Hydroxybutyryl groups also were identified in lipopolysaccharides of 5 strains of this genus.
SDS-PAGE of the lipopolysaccharides was presented in the paper. According to these results two core types exist in H. alvei . |
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| Keywords: | Lipopolysaccharide Hafnei alvei SDS-PAGE analysis |
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