Biodegradation of fluoroanilines by the wild strain Labrys portucalensis |
| |
| Institution: | 1. CBQF – Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal;2. CEQUIMED-UP, Laboratório de Química Orgânica e Farmacêutica, Departamento Ciências Químicas, Faculdade de Farmácia da Universidade do Porto, Rua Jorge Viterbo Ferreira n° 228, 4050-313 Porto, Portugal;1. Chemical Engineering Department, Jadavpur University, Kolkata 700 032, India;2. Chemical Engineering Department, Heritage Institute of Technology, Kolkata 700 107, India;1. Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005, Porto, Portugal;2. Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629 HZ, Delft, the Netherlands;1. CEB, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal;2. Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005, Porto, Portugal;3. Instituto Politécnico de Coimbra, ISEC, DEQB, Rua Pedro Nunes, Quinta da Nora, 3030-199, Coimbra, Portugal;4. Instituto de Investigação Aplicada, Laboratório SiSus, Rua Pedro Nunes, Quinta da Nora, 3030-199, Coimbra, Portugal;1. Biology Department and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal;2. Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital 172, 4200-374 Porto, Portugal;1. Department of Environmental Engineering, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, PR China;2. South China Institute of Environmental Sciences, The Ministry of Environment Protection, Guangzhou 510655, PR China;3. School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China |
| |
| Abstract: | Aniline and halogenated anilines are known as widespread environmental toxic pollutants released into soil and water. In contrast to aniline, which is rapidly metabolized via catechol, halosubstituted anilines are more resistant to microbial attack. A fluorobenzene-degrading bacterium, Labrys portucalensis strain F11, was tested under different culture conditions for the degradation potential towards 2-, 3- and 4-fluoroaniline (2-, 3- and 4-FA). Strain F11 was able to use FAs as a source of carbon and nitrogen however, supplementation with a nitrogen source improved substrate consumption and its dehalogenation extent. When F11 cells were previously grown on fluorobenzene (FB), higher biodegradation rates were achieved for all isomers. Complete 2-FA biodegradation with stoichiometric fluoride release was achieved when FB-induced cells were used. On the other hand, the degradation of 3- and 4-FA was characterized by incomplete defluorination of the target compounds suggesting accumulation of fluorinated intermediates. F11 cultures simultaneously supplied with FB and the fluorinated anilines showed a concomitant degradation of both substrates, suggesting co-metabolic biodegradation. To our knowledge, this is the first time that biodegradation of 2- and 3-FA as a sole carbon and nitrogen source and co-metabolic degradation of FA isomers in the presence of a structural analogous compound is reported. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
