Electrophoresis of periodontal pathogens in poly(ethyleneoxide) solutions with uncoated capillary |
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| Authors: | Zhenqing Li Ronglin Yang Qi Wang Dawei Zhang Songlin Zhuang Yoshinori Yamaguchi |
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| Institution: | 1. Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China;2. Photonics and Biomedical Research Institute, Department of Physics, Faculty of Science, East China University of Science and Technology, Shanghai 200237, China;3. Department of Applied Physics, Graduate School of Engineering, Osaka University, Suita City, Osaka 565-0871, Japan |
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| Abstract: | Periodontitis is a prevalent inflammatory disease caused by different species of anaerobic bacteria such as Porphyromonas gingivalis (P.g), Treponema denticola (T.d), and Tannerella forsythia (T.f). We compared the separation result of DNA ladders in hydroxyethyl cellulose, poly(ethyleneoxide) (PEO), and polyethylene glycol and analyzed the effect of polymer concentration, electric field, and temperature of the background electrolyte on the separation performance. Results demonstrated that there was a linear relationship (R = 0.942) for 100 to 700 bp of DNA and its migration time. Finally, the polymerase chain reaction products of P.g, T.d, and T.f were successfully identified within 8.5 min in 0.5% PEO with uncoated capillary. |
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| Keywords: | Capillary electrophoresis Polymerase chain reaction Periodontal pathogens |
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