Copper alone, but not oxidative stress, induces copper-metallothionein gene in Neurospora crassa |
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Authors: | Kumar K Satish Dayananda S Subramanyam C |
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Institution: | Department of Biochemistry, University College Science, Osmania University, Hyderabad 500 007, India. |
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Abstract: | Two metal response elements, flanking an antioxidant response element, were identified in regions upstream (-3730 bp) to copper metallothionein (CuMT) gene of Neurospora crassa. Presence of copper in culture media, but not of pro-oxidants like H2O2 or menadione, induced CuMT gene expression that could not be completely abolished by antioxidants such as N-acetyl cysteine and ascorbic acid. Gel shift assays revealed the ability of nuclear extracts from copper induced cultures to bind PCR-amplified metal response or antioxidant response elements. Similar observations could not be made with cultures exposed either to pro-oxidants or antioxidants. These results differentiate between CuMT gene induction by copper from antioxidant functions associated with the identified upstream elements. |
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Keywords: | Antioxidants Copper Metallothionein Oxidative stress |
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