| 猕猴桃属种间体细胞杂种 |
| |
| 引用本文： | 肖尊安,韩碧文.猕猴桃属种间体细胞杂种[J].Acta Botanica Sinica,1997,39(12):1110-1117. |
| |
| 作者姓名： | 肖尊安 韩碧文 |
| |
| 作者单位： | 北京师范大学生物系，中国农业大学生物学院 北京 100875，北京 100094 |
| |
| 基金项目： | The research is supported by grant from the National Natural Science Foundation of China. |
| |
| 摘 要： | 利用PEG融合方法,分别进行了中华猕猴桃(Actinidia chinensis var.chinensis)(2n=2x=58)子叶愈伤组织来源的原生质体与美味猕猴桃(A.deliciosa var.deiciosa)(2n=6x=174)子叶愈伤组织原生质体、以及狗枣猕猴桃(A.kolomikta)(2n=2x=58)叶肉原生质体种间原生质体融合。结果表明:中华猕猴桃与美味猕猴桃融合的1个克隆和中华猕猴桃与狗枣猕猴桃融合的4个克隆的RAPD谱带分别具有双亲特异的DNA谱带;经流式细胞仪分析,前者细胞核倍性推测为8倍体,后者细胞核为3倍体、4倍体和5倍体。初步鉴定这5个克隆是猕猴桃属种间体细胞杂种。
|
| 关 键 词： | 中华猕猴桃 美味猕猴桃 狗枣猕猴桃 种间体细胞杂种 |
INTERSPECIFIC SOMATIC HYBRIDS IN ACTINIDIA |
| |
| Authors: | Xiao Zun-an and Han Bi-wen |
| |
| Abstract: | Protoplasts isolated from cotyledon-derived calli of Actinidia chinensis var. chinensis (2n = 2x=58) were fused by the PEG method with cotyledon-callus protoplasts of A. deliciosa var. deliciosa (2n = 6x = 174) or with mesophyll protoplasts of A. kolomikta (2n = 2x = 58), respectively. Randomly amplified polymorphic DNA (RAPD) markers and flow cytometry was used to confirm the occurrence of somatic hybrids. RAPD results with some primers surveyed indicated that one clone (A. chinensis A. deliciosa) and four clones (A. chinensis A. kolomikta) had RAPD banding patterns which combined the parental banding profiles. Ploidy levels of the (A. chinensis A. deliciosa) clone were deduced as octoploid (2n = 8x), and the (A. chinensis A. kolomikta) clones were tetraploid (2n = 4x), triploid (2n = 3x) or pentaploid (2n = 5x). The clones were confirmed as interspecific somatic hybrids in Actinidia. |
| |
| Keywords: | Actinidia chinensis Actinidia deliciosa Actinidia kolomikta interspecific somatic hybrid |
| 本文献已被 CNKI 维普 等数据库收录！ |
