西双版纳小耳猪群体遗传结构的RFLP分析

目的　对西双版纳小耳猪的群体遗传结构进行RFLP分析。方法　采用ECL核酸直接标记和检测系统，以HRP标记ɑ-珠蛋白-3’HVR多基因座小卫星探针，对西双版纳小耳猪的Hinf Ⅰ或HaeⅢ酶切片段进行Southern杂交，以化学发光法进行检测，获得了清晰可辨的、具有高度多态性的DNA指纹图谱。结果　2　kb以上的谱带数在6～15条之间，HinfⅠ酶切产生的图带数低于HaeⅢ。JB亚系内805、807家系和JS亚系内111、121、121、151家系不同个体间的相似系数分别为0.94±0.09、0.85±0.08、0.84±1.7、0.78±1.6、0.83±0.42、0.87±0.07，显著高于各家系间的相似系数，JB亚系内的805家系和JS亚系内的151家系不同个体间相似系数较大，分别为0.94和0.87。结论　西双版纳小耳猪近交程度较高，个体间差异较小，均一性较强。

RELP Analysis of Genetic Structure in Banna Minipig Inbred Lines

Objective RFLP analysis was co nducted to the genetic structureof Banna minipig inbred lines.Methods Clearly and highly polymorphic DNA f in gerprints from Banna miniature pig were obtained by southern hybridization with a nonisotopically HRP(HorseRadish Peroxidase) labelled multiloci minisatellite p robe ɑ-globin 3'HVR，and detected by enhanced luminescence. Results The fragment number of over 2kb ranged from 6 to 15，a n more fragments resulted from the digestion of HinfⅠthan those of HaeⅢ.The s imilarity coefficients of various individuals from family 805，807 in substrain J B and family111，121，131，151 in substrain JS were 0.94±0.09，0.85±0.08， 0. 84±1.7，0.78±1.6，0.83±0.42，0.87±0.07，which was higher than that be tween families.Furtherly，similarity coefficients of family 805 in s ubstrain JB and famil y 151 in substrain JS were 0.94 and 0.87 respectively，which were higher than o thers.Conclusion The two families have the higher inbreeding degree，with the smaller genetic variation among individuals and the better homogeneity.