Estrogen-mediated induction of a vitellogenin-specific nonhistone chromatin protein in the male chicken liver |
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Authors: | Tatsuo Nakayama Maki Irikura Yoshiko Setoguchi Masami Nakayama Mitsuyoshi Mochizuki and Kikuo Ogata |
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Institution: | (1) Department of Biochemistry, Miyazaki Medical College, Kiyotake, 889-16 Miyazaki;(2) Department of Biochemistry, Niigata University School of Medicine, Asahimachi, 951 Niigata, Japan |
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Abstract: | Summary Non-histone chromatin proteins prepared from the livers of estrogen-treated and nontreated male chickens were compared by reverse-phase high performance liquid chromatography (RP-HPLC), followed by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results revealed that the hormone-treated male liver chromatin contained a specific protein corresponding to the vitellogenin-specific protein previously purified from the liver of egg-laying hens (Nakayama 1985). The chicken protein, purified further by gel-filtration high performance liquid chromatography (GF-HPLC), showed specific binding activity to DNA fragments carrying a part of the vitellogenin gene. On the basis of similarities in the elution patterns from GF-HPLC and RP-HPLC as well as in the mobility on SDS-PAGE, we concluded that this hormone-induced protein in the male chicken liver was identical to the vitellogenin-specific protein identified in the hen liver, and assumed it to be a specific regulatory protein for the vitellogenin gene expression. The amino acid composition of this chicken protein has been determined. |
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