Identification and characterization of IL-1 receptor-associated kinase-4 (IRAK-4) in half-smooth tongue sole Cynoglossus semilaevis |
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Authors: | Yu Yan Zhong Qiwang Li Chunmei Jiang Liming Wang Yanan Sun Yeying Wang Xubo Wang Zhigang Zhang Quanqi |
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Affiliation: | a Department of Life Science, Huainan Normal University, PR China b College of Marine Life Science, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China c Department of Bioscience and Engineering, Jiangxi Agricultural University, PR China |
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Abstract: | As a crucial component in TLR/IL-1R signaling pathways, IRAK-4 plays a central role in innate and adaptive immunity. In the present study, the cDNA of IRAK-4 was cloned for the first time from half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of csIRAK-4 was 2149 bp and contained a 168 bp 5′ UTR, a 580 bp 3′ UTR and a 1401 bp CDS. The predicted protein sequence of csIRAK-4 had two typical domains, a death domain (DD) at the N terminus and a serine/threonine/tyrosine protein kinase domain (STYKc) at the C terminus. RT-PCR showed that csIRAK-4 mRNA was detected in all tested tissues, especially in immune-related organs, gonads and brain. After injected with inactivated Vibrio anguillarum, the expressions of csIRAK-4 were up-regulated significantly (P < 0.05) in spleen and head kidney. During development, csIRAK-4 was expressed at all selected stages and low-level expression was detected at metamorphosis. Taken together, the present study indicated that csIRAK-4 played a crucial role in immune responses and might be involved in the process of development. |
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Keywords: | IRAK-4 IRAK family Cynoglossus semilaevis Vibrio anguillarum Gene expression |
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