| 五唇兰精细胞的分离 |
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| 引用本文： | 伍成厚,赵玉辉,杨延红,田惠桥.五唇兰精细胞的分离[J].热带亚热带植物学报,2012,20(4):393-398. |
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| 作者姓名： | 伍成厚 赵玉辉 杨延红 田惠桥 |
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| 作者单位： | 1. 厦门大学生命科学学院,福建厦门361005
2. 广州市园林科学研究所,广州510405 |
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| 基金项目： | 广东省科技计划项目(2007B020801007); 广州市科技计划项目(11A63030233)资助 |
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| 摘 要： | 五唇兰(Doritis pulcherrima)具二胞花粉,授粉后1 d即有花粉开始萌发,授粉后5 d开始有生殖细胞完成有丝分裂形成一对精细胞。通过人工授粉使花粉管在子房内发育,再利用花粉管直接爆破,成功分离出五唇兰的精细胞。成对的2个精细胞在直径大小、荧光强弱上均显示出较大差异,预示2个精细胞具有不同的前途。
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| 关 键 词： | 五唇兰 生殖细胞 精细胞 花粉管爆破 精细胞分离 |
| 收稿时间： | 2011/10/8 0:00:00 |
| 修稿时间： | 2011/12/15 0:00:00 |
Isolation of Sperm Cells in Doritis pulcherrima |
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| WU Cheng-hou,ZHAO Yu-hui,YANG Yan-hong and TIAN Hui-qiao.Isolation of Sperm Cells in Doritis pulcherrima[J].Journal of Tropical and Subtropical Botany,2012,20(4):393-398. |
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| Authors: | WU Cheng-hou ZHAO Yu-hui YANG Yan-hong and TIAN Hui-qiao |
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| Institution: | 1. School of Life Sciences, Xiamen University, China;2. Guangzhou Institute of Landscape Gardening, China;School of Life Sciences, Xiamen University, China;School of Life Sciences, Xiamen University, China;School of Life Sciences, Xiamen University, China |
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| Abstract: | The mature pollen of Doritis pulcherrima is two-celled. The pollen began to germinate after pollinated one day. After pollinated five days, the division of generative cell occurred in pollen tube and formed a pair of sperm cells. The pollen tubes were induced to development in the ovary after artificial pollination and sperm cells were isolated from the tubes by immediately blowing up in a broken solution containing 5%-12% mannitol. The two sperm cells isolated were dimorphism, one was big and the other small, and the fluorescent intensity was distinctly different. It suggested that both sperm cells would have different futures. |
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| Keywords: | Doritis pulcherrima Generative cell Sperm cell Pollen tube blow up Sperm cell isolation |
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