Continuous penicillin G hydrolysis in an electro-membrane reactor with immobilized penicillin G acylase |
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Authors: | Zadrazil Ales Chmelíková Růzena Hasal Pavel |
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Institution: | (1) Department of Chemical Engineering & Center for Nonlinear Dynamics of Chemical and Biological Systems, Institute of Chemical Technology, Prague, Technická 5, 166 28 Praha 6, Czech Republic |
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Abstract: | Penicillin G (2%, w/v in phosphate buffer, pH 8) was hydrolysed in a flow-through, miniature electro-membrane reactor with the penicillin G acylase immobilized in 5% (w/v) polyacrylamide (diam. 10 mm, thickness 2.6 mm, enzyme activity 24 U ml–1). The conversion of penicillin G increased from 0.15 to almost 0.5 when the electric current applied to the reactor was changed from –600 to +600 A/m2 with a substrate residency of 1 h.
Symbols and abbreviations
c
j
p & concentration of component j in product stream (M) c
j
s & concentration of component j in substrate stream (M) c
s
o & substrate concentration at reactor inlet (M) C
j
p=c
j
p/c
S
0 & scaled concentration of component j in product stream C
j
s=c
j
s/c
S
0 & scaled concentration of component j in substrate stream i & electric current density (A/m2) j & reaction component, j P, Q or S P & main reaction product (6-aminopenicillanic acid) PGA & penicillin G acylase Q & side reaction product (phenylacetic acid) S & substrate (penicillin G) Y
s=C
P
s+C
P
p & substrate conversion & mean residence time of substrate and product streams in reactor (h) =C
Q
s+C
Q
p+C
S
s+C
S
s & check-sum of scaled concentrations =C
P
p/(C
P
s+C
P
p) & separation factor of 6-aminopenicillanic acid (0 1) |
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Keywords: | electric field immobilized enzyme mass transfer membrane reactor penicillin acylase |
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