| 反义c-fos寡脱氧核苷酸对hCG诱导大鼠黄体细胞孕酮和雌二醇生成的影响 |
| |
| 引用本文： | 郑月慧,况海斌,方廉,吴绮明. 反义c-fos寡脱氧核苷酸对hCG诱导大鼠黄体细胞孕酮和雌二醇生成的影响[J]. 动物学报, 1999, 45(2): 2 |
| |
| 作者姓名： | 郑月慧 况海斌 方廉 吴绮明 |
| |
| 作者单位： | 1. 江西医学院生理学教研室,南昌,330006
2. 江西医学院病理学教研室,南昌,330006 |
| |
| 摘 要： | 采用离体孵育大鼠黄体细胞的方法，观察了反义c-fos寡脱氧核苷酸(反义c-fosODN)对hCG诱导的黄体细胞孕酮(P)和雌二醇(E
|
| 关 键 词： | 大鼠 离体黄体细胞 孕酮 雌二醇 反义寡脱氧核苷酸 |
EFFECT OF C-FOS ANTISENSE OLIGODEOXYNUCLEOTIDE ON THE HCG-INDUCED PROGESTERONE AND ESTRADIOL PRODUCTION IN RAT LUTEAL CELLS |
| |
| ZHENG Yue-Hui,KUANG Hai-Bin,FANG Lian,WU Qi-Ming. EFFECT OF C-FOS ANTISENSE OLIGODEOXYNUCLEOTIDE ON THE HCG-INDUCED PROGESTERONE AND ESTRADIOL PRODUCTION IN RAT LUTEAL CELLS[J]. Acta Zoologica Sinica, 1999, 45(2): 2 |
| |
| Authors: | ZHENG Yue-Hui KUANG Hai-Bin FANG Lian WU Qi-Ming |
| |
| Abstract: | The effects of c-fos antisense oligodeoxynucleotides (antisense c-fos ODN) on the hCG-induced progesterone and estradiol production in isolated rat luteal cells and the effect of cAMP and Ca2+on c-fos oncoprotein in luteal cells were investigated. Antisense cfos ODN inhibited the hCG-induced progesterone and estradiol production in a dose-dependent manner, and the percentages of luteal cells stained for c-fos protein were also decreased significantly. Nonsense tat ODN had no similar effects on luteal cells. Further experiments showed that 10-4mol/L dbcAMP increased the percentage of luteal cells stained for c-fos protein,and increased the hCG-induced P and E, production. When combined with antisense c-fos ODN, dbcAMP reversed the inhibition of hCG-induced P and E, production, and increased the percentage of luteal cells stained for c-fos protein. However verapamil (10-6mol/L) had opposite effects on c-fos expression and hCG-induced P and E, production as compared with dbcAMP. |
| |
| Keywords: | c-fos |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
