The hapten structure of a developmentally regulated glycolipid antigen (SSEA-1) isolated from human erythrocytes and adenocarcinoma: a preliminary note |
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| Authors: | S Hakomori E Nudelman S Levery D Solter B B Knowles |
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| Institution: | 1. Biochemical Oncology, Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA;2. University of Washington, Seattle, WA 98104, USA;3. the Wistar Institute, Philadelphia, PA 19104 USA |
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| Abstract: | Glycolipid antigen reacting to the monoclonal antibody directed to the developmentally regulated antigen SSEA-1 was isolated from human erythrocytes and colonic adenocarcinoma. The antigens have the Lex (Galβl→4Fucα]→3]GlcNAcβl→R) or Ley (Fucαl→2Galβl→4Fucαl→3]GlcNAcβl→R) structure at the termini of the branched polylactosaminolipid. In addition, a novel polyfucosyl structure locating exclusively at the internal GlcNAc was detected in the tumor antigen. The antibody reacts with a simple monovalent Lex glycolipid (Galβl→4Fucαl→3]GlcNAcβl→3Galβl→4Glcβl→Cer) previously isolated from colonic carcinoma when presented at a high density on liposomes. The antibody therefore may react to the bivalent or multivalent Lex or Ley structure. |
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| Keywords: | TLC thin-layer chromatography DCP dicetylphosphate DMPC dimyristoylphosphatidylcholin SSEA-1 stage-specific embryonic antigen-1 (1) |
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