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| 基于糖显色法测定鼠李糖脂的比例、含量 | |
| 引用本文： | 盖立学,宋考平. 基于糖显色法测定鼠李糖脂的比例、含量[J]. 生物技术, 2010, 20(2): 33-37 |
| 作者姓名： | 盖立学 宋考平 |
| 作者单位： | 1. 大庆石油学院石油工程学院,黑龙江大庆163318;大庆油田有限责任公司,黑龙江大庆163453 2. 大庆石油学院石油工程学院,黑龙江大庆,163318 |
| 摘 要： | 目的:确定微生物培养液中鼠李糖脂的组成、含量和鼠李糖脂在电喷雾质谱(ESI-MS)中的离子化效率.方法:用薄层色谱分离并结合ESI-MS分析培养液中的糖脂产物、测定样品中单鼠李糖脂和双鼠李糖脂比例及离子化强度.结果:根据苯酚-硫酸法和蒽酮光度法中糖及糖脂与吸光度的定量关系[A=0.0103X+0.0465(X为鼠李糖量,μg),A=0.0043X+0.0446(X为鼠李糖脂量,μg)],用化学计量方法确定了糖脂含量84.8%,其中单鼠李糖脂的质量分数0.344,双鼠李糖脂的质量分数0.656,并基于电喷雾质谱中的离子强度和测定的浓度计算了鼠李糖脂的离子化效率,双鼠李糖脂的钠离子化效率仅为单鼠李糖脂钠离子化效率的50%.结论:可用于定量分析单双糖脂及评价鼠李糖脂的生产. |
| 关 键 词： | 单鼠李精糖脂 双鼠李糖糖脂 鼠李糖脂 电喷雾质谱 含量 离子化效率 |
Ratio and Content of Rhamnolipid Determined using Sugar Development Process |
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| GAI Li-xue,,SONG Kao-ping. Ratio and Content of Rhamnolipid Determined using Sugar Development Process[J]. Biotechnology, 2010, 20(2): 33-37 | |
| Authors: | GAI Li-xue SONG Kao-ping |
| Affiliation: | GAI Li-xue1,2,SONG Kao-ping1(1.College of Petroleum Engineering,Institute of Daqing Petroleum,Daqing 163318,China,2.Daqing Oil Field Corp.Ltd.,Daqing 163453,China) |
| Abstract: | Objective:To determine the composition and contents of the rhamnolipids in microbial culture meadia,and the ionization efficiency of rharmnolipids in Electro Spray Ionization Mass Spectrometry(ESI-MS).Method:Thin Layer Chromatography(TLC)combined with ESI-MS were used to analysize the composition of rhamnolipids,the ratio of single rhamnose glycolipid and double rhamnose glycolipid,and ionization strength.Result:Based on the relationship of rhamnose and glycolipid with absorbance in the methods of phenol-vi... |
| Keywords: | single rhamnose glycolipid double rhamnose glycolipid rhamnolipid ESI - MS content ionization efficiency |
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