大米草对赤潮藻的抑制作用及其抑藻物质的分离鉴定

以赤潮异弯藻和海洋原甲藻为研究对象,研究了海洋滩涂植物大米草对两种赤潮微藻的抑制作用及抑藻物质.结果表明：大米草对赤潮异弯藻的影响表现为高浓度抑制生长,低浓度则有一定的促进作用,大米草新鲜组织、干粉末和提取物浓度分别为4.8、0.8和0.5 mg·ml^-1以上时对赤潮异弯藻有致死作用；大米草对海洋原甲藻则表现出明显的抑制作用,且大米草新鲜组织、干粉末和提取物浓度分别为9.6、1.6和1.25 mg·ml^-1以上时对海洋原甲藻有致死作用.对大米草提取物进行抑藻活性物质的分离纯化,从中分离鉴定了2个具有抑藻活性的黄酮糖苷类化合物：异鼠李素-3-O-槐二糖-7-O-鼠李糖苷和丁香亭-3-O-半乳糖苷.

Inhibitory effects of Spartina anglica on Heterosigma akashiwo and Prorocenrum micans and the isolation and identification of the algicidal compounds

Taking Heterosigma akashiwo and Prorocenrum micans as test materials,the algicidal activity of Spartina anglica against the growth of the two harmful microalgae were investigated,with the algicidal compounds isolated and identified.Lower concentration S.anglica stimulated the growth of H.akashiwo,while high concentration S.anglica inhibited the H.akashiwo growth significantly.The lethal concentration of S.anglica fresh tissue,dry powder,and extracts to H.akashiwo was 4.8 mg·ml~(-1),0.8 mg·ml~(-1),and 0.5 mg·ml~(-1),respectively.All test concentration S.anglica had obvious inhibitory effects on the growth of P.micans,and the lethal concentration of S.anglica fresh material,dry powder,and extracts was 9.6 mg·ml~(-1),1.6 mg～ml~(-1),and 1.25 mg·ml~(-1),respectively.Two algicidal compounds in S.anglica were isolated,and identified as isohamnetin-3-O-sophoroside-7-O-rhamnoside and syringetin-3-O-galactoside.