Carpospore early development and callus-like tissue induction of <Emphasis Type="Italic">Chrysymenia wrightii</Emphasis> (Rhodymeniaceae,Rhodophyta) under laboratory conditions |
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Authors: | Fengjuan Zhao Ziguo Zhao Aihua Wang Jidong Liu Delin Duan |
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Institution: | (1) Shandong Key Laboratory of Eco-environmental Science for Yellow River Delta, Binzhou University, Binzhou, 256600, China;(2) Department of Life Science, Binzhou University, Binzhou, 256600, China;(3) School of Life Science, Qingdao Agriculture University, Qingdao, 266109, China;(4) Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; |
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Abstract: | Morphological and culture studies of germlings derived from carpospores of Chrysymenia wrightii (Harvey) Yamada were carried out under various treatments combining temperature and irradiance. Basal, main, and tip branches
were applied for inducing callus-like tissue. Focus was on how carpospores develop into germlings, how callus-like tissues
are induced from explants, and how temperature and irradiance affect carpospore germination and discoid crust growth. Results
show that carpospore development can be divided into three stages: division stage, discoid crust stage, and erect juvenile
germling stage. Discoid crusts, even more than ten, might coalesce into a big discoid crust, and then developed into germlings.
Filamentous fronds, formed on the rims of discoid crusts, exhibited in self-existence or co-existence form with germlings,
could form spherical tufts if cultured separately. Filamentous callus-like tissues appeared on the tip branches after 13 days.
PES is suitable for filament induction and culture, and filaments have potential use in germplasm preservation and vegetative
propagation. Temperature (10, 15, 20, 25°C) and irradiance (8 and 36 μmol photons m−2 s−1) significantly influenced carpospore germination rate and discoid crust diameter. Carpospores germinated normally under 36 μmol
photons m−2 s−1, 15~25°C, and maximum growth of discoid crusts was at 25°C, 36 μmol photons m−2 s−1; 10°C and 8 μmol photons m−2 s−1 did not favor carpospore germination or discoid crust growth. |
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