Expression of the Ruminococcus flavefaciens cellodextrinase gene in Saccharomyces cerevisiae |
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| Authors: | Pierre van Rensburg Willem H van Zyl Isak S Pretorius |
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| Institution: | (1) Department of Microbiology and Institute for Biotechnology, University of Stellenbosch, 7600 Stellenbosch, Republic of South Africa |
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| Abstract: | Summary A recombinant strain of Saccharomyces cerevisiae secreting bacterial cellodextrinase was constructed. The Ruminococcus flavefaciens cellodextrinase gene (celA) was inserted between a yeast expression-secretion cassette and yeast gene terminator, and cloned into a yeast-centromeric shuttle vector. Enzyme assays revealed growth-associated production of biologically active cellodextrinase by S. cerevisiae transformants. |
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