Amino acid sequence of the minor isomorph of the crustacean hyperglycemic hormone (CHH-II) of the Mexican crayfish Procambarus bouvieri (Ortmann): Presence of a d-amino acid |
| |
| Authors: | Manuel B Aguilar Daniel Soyez Rocco Falchetto David Arnott Jeffrey Shabanowitz Donald F Hunt Alberto Huberman |
| |
| Institution: | aDepartment of Biochemistry, Instituto Nacional de la Nutrición “S. Zubirán,” Vasco de Quiroga 15, Tlalpan, Mexico City 14000, Mexico;bLaboratoire de Biochimie et Physiologie du Développement, URA CNRS 686, Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris cedex, France;cDepartment of Chemistry, Biological Mass Spectrometry Laboratory, University of Virginia, McCormick Road, Charlottesville, VA 22901 USA;dDepartment of Chemistry, University of Virginia, McCormick Road, Charlottesville, VA 22901 USA;eDepartment of Pathology, University of Virginia, McCormick Road, Charlottesville, VA 22901 USA |
| |
| Abstract: | The primary structure of the neurohormone crustacean hyperglycemic hormone (CHH-II) was determined by means of enzymatic digestions, manual Edman degradation, and mass spectrometry. CHH-II is a 72 residue peptide (molecular mass 8388 Da), with six cysteines forming three disulfide bridges that connect residues 7–43, 23–39, and 26–52. The peptide has blocked N- and C-termini, and lacks tryptophan, histidine, and methionine. The CHH-I and CHH-II of Procambarus bouvieri have identical sequences and elicit levels of hyperglycemia that are not distinguishable. The difference between the two isomorphs consists in a posttranslational modification of a l-Phe in CHH-I to a d-Phe in CHH-II at the third position from the N-terminus. |
| |
| Keywords: | Crustacean hyperglycemic hormone Neuropeptide Isomorph Sequence Procambarus bouvieri Crayfish d-Amino acids" target="_blank">d-Amino acids Posttranslational modification |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
