Sequence analysis of a salt tolerant metagenomic clone |
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Authors: | Raj Kishor Kapardar Ravi Ranjan Munish Puri Rakesh Sharma |
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Institution: | (1) Department of Microbiology and Immunology, Life Sciences Centre, 2350 Health Sciences Mall, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada |
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Abstract: | Metagenome represent an unlimited resource for discovery of novel genes. Here we report, sequence analysis of a salt tolerant
metagenomic clone (6B4) from a pond water metagenomic library. Clone 6B4 had an insert of 2254 bp with G+C composition of
64.06%. DNA sequence from 6B4 showed homology to DNA sequences from pro-teobacteria indicating origin of 6B4 metagenomic insert
from a yet uncharacterized proteobacteria. Two encoded proteins from clone 6B4 showed match with ATP-depen-dent Clp protease
adaptor protein (ClpS) and phasin, while two truncated encoded proteins showed match with poly-3-hydroxybutyrate synthase
and permease. Clp complex is known to play a role in stress tolerance. Expression of ClpS from metagenomic clone is proposed
to be responsible for salt tolerance of the metagenomic clone 6B4. |
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Keywords: | Metagenomic Metagenome Salt tolerance ClpS |
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