|
|||||
|
|
Stable RNA Interference in Spodoptera frugiperda Cells by a DNA Vector-based Method |
|
| Authors: | Chih-Chien Lin John T -A Hsu Kai-Ling Huang Hung-Kuan Tang Yiu-Kay Lai |
| Institution: | (1) Department of Life Science and Institute of Biotechnology, National Tsing Hua University, Hsinchu, 30013, Taiwan, R.O.C.;(2) Division of Biotechnology and Pharmaceutical Research National Health Research Institutes, Miaoli, 350, Taiwan, R.O.C.;(3) Department of Chemical Engineering, National Tsing Hua University, Hsinchu, 30013, Taiwan, R.O.C. |
| Abstract: | Double-stranded RNA (dsRNA)-mediated interference (RNAi) is a powerful tool for silencing of gene expression in many organisms. To establish a DNA vector-based method for stable RNAi in Spodoptera frugiperda cells (Sf9), we created a stably transfected Sf9 cell line to express large dsRNA fragment targeting to silence the firefly luciferase gene (luc). The luc dsRNA specifically and stably suppressed the baculovirus-mediated luciferase expression. Thus, gene silencing in Sf9 cells was achieved using DNA vectors similar to the facile design described in this study. Received 21 September 2005; Revisions requested 5 October 2005; Revisions received 22 November 2005; Accepted 25 November 2005 |
| Keywords: | baculovirus double-stranded RNA RNA interference Spodoptera frugiperda stable RNAi |
| 本文献已被 PubMed SpringerLink 等数据库收录！ | |