Relationship between the expression of class I antigen and reactivity of chicken thymocytes |
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Authors: | R Sgonc K Hála G Wick |
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Institution: | (1) Institute for General and Experimental Pathology, University of Innsbruck, Medical School, Fritz-Pregl-Strasse 3, A-6020 Innsbruck, Austria |
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Abstract: | The expression of major histocompatibility complex (MHC) class I antigens in ontogenesis and the distribution of B-F+ cells, defined by means of a monoclonal antibody, were studied by indirect membrane immunofluorescence tests on suspensions of thymus, bursa, spleen, peripheral blood lymphocytes (PBL) and red blood cells (RBC) from 18-day-old chicken embryos and chickens from 1–90 days after hatching. At 18 days of incubation and at the first day after hatching, RBC, PBL, and the cells from bursa and thymus are negative. The percentage of positive PBL and bursal cells increases up to 9 days after hatching. By 2 weeks after hatching almost 100 % of the RBC, PBL, bursa, and spleen cells were positive whereas the thymus showed only 20% positive cells. Analysis on 4-m-thick, frozen acetone-fixed tissue sections of thymus showed that medullary cells are positive, while the cortical area is negative. The graft-versus-host (GvH) competence of these thymus subpopulations was compared after sorting by the fluorescence-activated cell sorter and injection into MHC incompatible embryos. GvH reactivity was associated primarily with the B-F+ population. Double staining studies with peanut agglutinin (PNA)-fluorescein isothiocyanate and a rabbit-anti-Ig tetramethyl isothiocyanate-conjugate proved that the PNA– thymocytes are identical with B-F+ thymocytes.Abbreviations used in this paper: FACS
fluorescence-activated cell sorter
- FCS
fetal calf serum
- FITC-Ig
fluorescein isothiocyanate-conjugated immunoglobulin
- GvH
graft-versus-host
- HAT
hypoxanthineaminopterin-thymidine
- HBSS
Hanks' balanced salt solution
- IIF
indirect immunofluorescence
- MCA
monoclonal antibody
- MHC
major histocompatibility complex
- NWL
normal white Leghorn
- OS
Obese strain
- PBL
peripheral blood lymphocytes
- PBS
phosphate-buffered saline
- PNA
peanut agglutinin
- RBC
red blood cells
- TRITC-Ig
tetramethyl isothiocyanate-conjugated immunoglobulin |
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