Effects of macro elements and nitrogen source on adventitious root growth and ginsenoside production in ginseng (Panax ginseng C. A. Meyer) |
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Authors: | Kee-Won Yu Wen Yuan Gao Eun-Joo Hahn Kee-Yoeup Paek |
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Institution: | (1) Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763 Cheongju, Korea;(2) Hong Kong Institute of Biotechnology, Shatin N.T., Hong Kong |
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Abstract: | We investigated the effects on ginseng adventitious root growth and ginsenoside production when macro-element concentrations
and nitrogen source were manipulated in the culture media. Biomass growth was greatest in the medium supplemented with 0.5-strength
NH4PO3, whereas ginsenoside accumulation was highest (9.90 mg g-1 DW) in the absence of NH4PO3. At levels of 1.0-strength KNO3, root growth was maximum, but a 2.0 strength of KNO3 led to the greatest ginsenoside content (9.85 mg g-l). High concentrations of MgSO4 were most favorable for both root growth and ginsenoside accumulation (up to 8.89 mg g-1 DW). Root growth and ginsenoside content also increased in proportion to the concentration of CaCI2 in the medium, with the greatest accumulation of ginsenoside (8.91 mg g-1 DW) occurring at a 2.0 strength. The NH4/NO3
-- ratio also influenced adventitious root growth and ginsenoside production; both parameters were greater when the NO3
- concentration was higher than that of NH4
+. Maximum root growth was achieved at an NH4
+/NO3
- ratio of 7.19/18.50, while ginsenoside production was greatest (83.37 mg L-1) when NO3
- was used as the sole N source. |
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Keywords: | bioreactor macro element NH4 +/NO3 - ratio nitrogen source |
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