The in vivo trypanocidal effect of the diterpene 5-epi-icetexone obtained from Salvia gilliesii |
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| Institution: | 1. Laboratorio de Biología y Fisiología Celular, Instituto de Histología y Embriología — CONICET, Facultad de Ciencias Médicas, UNCuyo, Argentina;2. Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET — Cátedra de Física Biomédica and Centro de Estudios e Investigación de la Enfermedad de Chagas y Leishmaniosis, Facultad de Ciencias Médicas, UNC, Córdoba, Argentina;3. INTEQUI-CONICET, FQByF, UNSL, San Luis, Argentina;1. CIBIO Research Centre in Biodiversity and Genetic Resources, InBIO, Universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, N° 7, 4485-661 Vairão, Vila do Conde, Portugal;2. Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre FC4 4169-007 Porto, Portugal;3. Institut de Biologia Evolutiva (CSIC-Universitat Pompeu Fabra), Passeig Maritím de la Barceloneta, 37-49, 08003 Barcelona, Spain;1. Chiang Mai Night Safari, Chiang Mai 50230, Thailand;2. Veterinary Diagnostic Laboratory, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand;3. Veterinary Central Laboratory, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand;4. Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand;5. Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand;1. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan;2. Department of Pre-clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand;3. Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand;4. The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand;5. Livestock and Wildlife Hospital, Faculty of Veterinary Science, Mahidol University (Kanchanaburi Campus), Saiyok District, Kanchanaburi 71150, Thailand;1. Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, People''s Republic of China, 430070;2. Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, People''s Republic of China, 430070;3. Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, People''s Republic of China, 430070;1. Aquatic Parasitology Laboratory, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University (SFAAS), Auburn, AL 36849, United States;2. Aquatic Parasitology Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences, 203 Swingle Hall, Auburn University, Auburn, AL 36849, United States |
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| Abstract: | The search for new compounds with trypanocidal activity is crucial for the treatment of Chagas' disease. Previous in vitro studies have shown that the diterpene 5-epi-icetexone (ICTX) is active against Trypanosoma cruzi. The aim of this work was to evaluate the effect of ICTX on the parasites in infected mice, in an experimental model that mimics the acute phase of the disease. Swiss albino mice were infected with T. cruzi and treated daily with 10 mg/kg/day ICTX (i.p.). Infected mice and mice injected with either saline or the vehicle DMSO were used as controls. Animals' survival and parasitemia were monitored once a week and histological studies were made at necropsy by the 5th week after infection. It was observed that the administration of ICTX increased the survival of mice infected, and induced a significant decrease in the parasitemia, as compared to controls. A similar protective effect was observed when animals were treated orally with benznidazole (BZN, used as a control of antiparasitic effect). By the 5th week post-infection, the presence of amastigote nests was observed within the fibers of the cardiac and skeletal muscle in controls, but not in animals treated with either ICTX or BZN. In addition, inflammatory infiltrates were observed in the tissues of controls, but not in animals treated with the drugs. We conclude that ICTX has an antiparasitic effect against T. cruzi, thus constituting an interesting option for the treatment of Chagas' disease, alone or combined with other drugs. |
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