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Plant Regeneration from Mesophyll Protoplasts of Several Nicotiana Species
Authors:JEAN-PIERRE BOURGIN  YVES CHUPEAU  CLAUDINE MISSONIER
Institution:Laboratoire de Biologie cellulaire, Département de Physiologie végétale, Institut National de la Recherche Agronomique, F 78000 Versailles, France
Abstract:In a search for model systems in plant cell genetics studies mesophyll protoplasts from eleven species of Nicotiana with low chromosome number (N. acuminata, N. alata, N. glauca, N. glutinosa, N. langsdorffii, N. longiflora, N. otophora, N. paniculata, N. plumbaginifolia, N. suaveolens, N. sylvestris) were shown to divide in a liquid culture medium. Plants were recovered from calli originating from protoplasts of all these species except N. glutinosa.
Keywords:
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