| 分子识别诱导的蛋白质和核酸多层膜的有序组装 |
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| 引用本文： | 谢文章,梁俊峰,隋森芳.分子识别诱导的蛋白质和核酸多层膜的有序组装[J].生物物理学报,1996(2). |
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| 作者姓名： | 谢文章 梁俊峰 隋森芳 |
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| 作者单位： | 清华大学生物科学与技术系生物膜与膜生物工程国家重点实验室 |
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| 摘 要： | 通过生物大分子之间的特异性结合，采用表面等离激元共振技术监测，报导了支撑于固体表面脂单层膜上进行的亲和素、生物素标记的质粒ＤＮＡ、以及从系统性红斑狼疮患者血清中获得的抗ＤＮＡ抗体多层膜的有序组装。这种生物大分子的组装技术可以用于生物传感器以检测特定的抗原抗体。
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| 关 键 词： | 分子组装，亲和素，表面等离激元共振，LB膜 |
MOLECULAR RECOGNITION INDUCED FORMATION OF PROTEIN/NUCLEIC ACID MULTILAYERS |
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| Xie Wenzhang,Liang Junfeng,Sui Senfang.MOLECULAR RECOGNITION INDUCED FORMATION OF PROTEIN/NUCLEIC ACID MULTILAYERS[J].Acta Biophysica Sinica,1996(2). |
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| Authors: | Xie Wenzhang Liang Junfeng Sui Senfang |
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| Abstract: | Based on the specific binding,we report in the present paper the molecular assembly of protein/DNA multilayers on a thin metal film.The first layer is biotin-lipid containing monolayer prepared by LB technique.The second and third layers are avidin and biotin labeled DNA respectively.Both layers are formed upon the very strong binding constant of biotin with avidin.The fourth layer is anti-DNA anitibody,which was extrated from the patients'serum of SLE(Systemic Lupus Erythematosus).The multilayer formation process was detected by surface plasmon resonance spectroscopy. |
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| Keywords: | Molecular recognition Avidin Surface plasmon resonance LB film |
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