Effect of alcohols on the association of photosynthetic fructose-1,6-bisphosphatase to thylakoid membranes |
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Authors: | A Rodriguez Andres J J Lazaro A Chueca R Hermoso J Lopez Gorge |
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Institution: | Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, E-18008 Granada, Spain. |
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Abstract: | Aliphatic alcohols have a positive effect on the assoociation of pea ( Pisum sativum L. cv. Lincoln) chloroplast fructose- 1,6-bisphosphatase (FBPase; EC 3.1.3.11) with thylakoid membranes. The alcohol concentration needed to obtain a fixed percentage of enzyme association decreased with increased length of the aliphatic chain of the alcohol; maximum binding was obtained when the lysis medium contained, in molar fractions (or v/v percentages): 48×10-4(T4 (2.4%), 26×10-3 (10%), 40×10-3 (15%), 76×10-3 (21%), and 13×10-2 (24%), of 1-butanol, 1-propanol, 2-propanol, ethanol, and methanol, respectively. A good correlation of binding with the octanol/water partition coefficient was observed. Since this coefficient constitutes a measure of hydrophobicity, we suggest that the binding of FBPase to the membranes occurs via hydrophobic clusters of both components. |
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Keywords: | Alcohols binding chloroplast membranes fructose-1 6-bisphosphatase pea Pisum sativum |
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