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| 沉香叶提取工艺及其抗氧化活性实验研究 | |
| 引用本文： | 林芳花,彭永宏,江顺,廖建良,叶海宇. 沉香叶提取工艺及其抗氧化活性实验研究[J]. 中国野生植物资源, 2011, 30(4): 35-37,40 |
| 作者姓名： | 林芳花 彭永宏 江顺 廖建良 叶海宇 |
| 作者单位： | 1. 惠州学院生命科学系/生物技术研究所,广东惠州,516007 2. 惠州龙发山农业发展有限公司,广东惠州,516001 |
| 基金项目： | 广东省自然科学基金项目(915063201000018); 惠州市科技项目(2010B20010005); 惠州学院自然科学项目(C5090208),惠州学院生物化学与分子生物重点学科资助(惠院科发[2009]41号) |
| 摘 要： | 采用单因素分析方法和正交试验法研究沉香叶提取工艺并评价沉香叶的体外抗氧化活性.沉香叶的最佳提取工艺为:以15倍量的60％乙醇为溶剂,超声提取40 min,提取1次.野生沉香叶和栽培沉香叶的氧化时间分别为(11.53±0.08)s和(9.98±0.16)s；超氧阴离子的清除率分别为(36.26±0.96)％和(35.67±1.25)％,总还原力的吸光度分别为0.188±0.008和0.129±0.011.该提取工艺简单、重复性好、提取液抗氧化活性强、工艺稳定、无污染；野生沉香叶和栽培沉香叶均具有较好的体外抗氧化活性. |
| 关 键 词： | 沉香 提取工艺 抗氧化 |
Experimental Study on the Extracting Process and Antioxidant Activity of Aquilaria sinensis(Lour.) Gilg.Leaf |
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| Lin Fanghua , Peng Yonghong , Jiang Shun , Liao Jianliang , Ye Haiyu. Experimental Study on the Extracting Process and Antioxidant Activity of Aquilaria sinensis(Lour.) Gilg.Leaf[J]. Chinese Wild Plant Resources, 2011, 30(4): 35-37,40 | |
| Authors: | Lin Fanghua Peng Yonghong Jiang Shun Liao Jianliang Ye Haiyu |
| Affiliation: | Lin Fanghua1,Peng Yonghong1,Jiang Shun1,Liao Jianliang1,Ye Haiyu2(1.Department of Life Science and Institute of Biotechnology,Huizhou University,Huizhou 516007,China,2.Huizhou Longfa Mountain Agricultural Development Co.,Ltd.,Huizhou 516001,China) |
| Abstract: | Both single factor analysis and orthogonal experimental method were used to optimize the extracting process for aquilaria sinensis（Lour.） Gilg.leaf,and then their antioxidant activity was also evaluated in vitro.As shown in the result,the best extracting process for aquilaria sinensis（Lour.） Gilg.leaf requesting 60% ethanol as solvent,15 times solvent volume,extraced one times by ultrasonic continuously for 40 min;the oxidation time for cultivation and wild aquilaria sinensis（Lour.） Gilg.leaf were 11.53±0.08（s）and 9.98±0.16（s）;the clearance percent for super oxide anion appeared（36.26±0.96）% and（35.67±1.25）%;and the absorbency showed 0.188±0.008 and 0.129±0.011 respectively.The conclusions could be drawn that the extraction technology has good properties on stronger antioxidant activity,and the method is simple,reproducible and pollution-free;both cultivation and wild aquilaria sinensis（Lour.） Gilg.Leaf have stronger antioxidant activity in vitro. |
| Keywords: | Aquilaria sinensis(Lour.) Gilg. extracting process antioxidant activity |
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