PACAP-38 is a chemorepellent and an agonist for the lysozyme receptor in Tetrahymena thermophila |
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Authors: | S R Mace J G Dean J R Murphy J L Rhodes H G Kuruvilla |
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Institution: | (1) Cedarville College, Department of Science and Mathematics, PO Box 601, Cedarville, OH 45314, USA e-mail: kuruvilh@cedarville.edu Tel.: +1-937-766-7606; Fax: +1-937-766-7631, US |
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Abstract: | Pituitary adenylate cyclase activating peptide (PACAP-38) is a peptide hormone which functions in many mammalian systems,
including the nervous and digestive systems. Using in vivo behavioral studies, we have found that this hormone functions as
a chemorepellent in Tetrahymena thermophila with an EC50 of 10 nM. Cells previously adapted to PACAP-38 were found to be adapted to lysozyme, and vice versa. Furthermore, the in
vivo behavioral activity of PACAP-38 was blocked by addition of the anti-lysozyme receptor antibody, 5545. Chemorepellent
activity of PACAP-38 was also inhibited by the addition of neomycin sulfate (inhibition constant K
i=0.080 μmol · l−1), a competitive inhibitor of lysozyme binding to its receptor. PACAP-38 is a more potent and specific agonist for the lysozyme
receptor than either intact lysozyme or CB2, a 24-amino acid fragment of lysozyme.
Accepted: 11 October 1999 |
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Keywords: | Chemorepellent PACAP-38 Tetrahymena Chemosensory transduction Behavioral adaptation |
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