Studies on H-Y antigen in different cell fractions of the testis during pubescence |
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Authors: | Maria Teresa Zenzes U Müller Ilse Aschmoneit U Wolf |
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Institution: | (1) Institut für Humangenetik und Anthropologie der Universität, Albertstr. 11, D-7800 Freiburg i. Br., Germany |
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Abstract: | Summary Various cell types of the rat testis during pubescence, including germ, Sertoli, and Leydig cells, were partially enriched. The fractions were tested for the presence, binding, and secretion of H-Y antigen. The main results are: Immature germ cells are H-Y antigen-negative until the late diploid stages, and late primary spermatocytes or spermatids become positive; the somatic cells of the gonad are positive at all ages examined (18 days old to adulthood). Secretion of H-Y antigen is restricted to the Sertoli cell fraction. Binding of externally supplied antigen takes place on Leydig cells; the Sertoli cell surface will be saturated because of active secretion; there is no binding to germ cells. Thus, immature germ cells seem to be the only H-Y antigen-negative cells of the male organism, and the Sertoli cells seem to be the only ones to secrete H-Y antigen. |
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