A linkage map with RFLP and isozyme markers for almond |
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Authors: | M A Viruel R Messeguer M C de Vicente J Garcia-Mas P Puigdomènech F Vargas P Arús |
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Institution: | (1) Departament de Genètica Vegetal, Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Carretera de Cabrils s/n, 08348 Cabrils (Barcelona), Spain;(2) Consejo Superior de Investigaciones Cientifícas (CSIC), Departament de Genètica Molecular, Centre d'Investigació i Desenvolupament de Barcelona, Jordi Girona Salgado 18-22, 08007 Barcelona, Spain;(3) Departament d'Arboricultura Mediterrània, Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Apartat 415, 43280 Reus (Tarragona), Spain |
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Abstract: | Inheritance and linkage studies were conducted with seven isozyme genes and 120 RFLPs in the F1 progeny of a cross between almond cultivars Ferragnes and Tuono. RFLPs were detected using 57 genomic and 43 cDNA almond clones. Eight of the cDNA probes corresponded to known genes (extensin, prunin (2), -tubulin, endopolygalacturonase, oleosin, actin depolymerizing factor and phosphoglyceromutase). Single-copy clones were found more frequently in the cDNA (65%) than in the genomic libraries (26%). Two maps were elaborated, one with the 93 loci heterozygous in Ferragnes and another with the 69 loci heterozygous in Tuono. Thirty-five loci were heterozygous in both parents and were used as bridges between both maps. Most of the segregations (91%) were of the 11 or 1111 types, and data were analyzed as if they derived from two backcross populations. Eight linkage groups covering 393 cM in Ferragnes and 394 in Tuono were found for each map. None of the loci examined in either map was found to be unlinked. Distorted segregation ratios were mainly concentrated in two linkage groups of the Ferragnes map. |
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Keywords: | Almond Prunus amygdalus Isozymes RFLPs Genetic maps |
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