Cellulose acetate electrophoresis of protein kinases: Detection of the active forms using various substrates |
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Authors: | Françoise Phan-Dinh-Tuy Anne Weber Joëlle Henry Dominique Cottreau Axel Kahn |
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Institution: | 1. Départment de Production de cellules humaines, Institut de Cancérologie et d''immunogénétique, 94804 Villejuif Cedex, France;2. Institut de Pathologie Moléculaire, Chu Cochin, 24 rue du Fg St Jacques, 75674 Paris Cedex 14, France |
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Abstract: | Electrophoresis on cellulose acetate strips was used to analyze protein kinases from normal rat liver. In addition to already well-characterized cAMP-dependent protein kinases type I and II and cAMP-independent casein kinases I and II, this method enabled the detection of several supplementary bands corresponding to kinases which were investigated according to their substrate specificity, activation by cAMP, and inhibition by the specific inhibitor of the catalytic subunit of cAMP-dependent protein kinases or by heparin. Using this rapid, sensitive, and resolutive electrophoretic method, different isozyme patterns could be obtained starting from minute amounts of different types of biological material. |
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Keywords: | To whom reprint requests should be sent |
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