Stabilizing effect of Zn2+ in native bovine rhodopsin |
| |
| Authors: | Park Paul S-H Sapra K Tanuj Koliński Michał Filipek Sławomir Palczewski Krzysztof Muller Daniel J |
| |
| Institution: | Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, USA. paul.park@case.edu |
| |
| Abstract: | Single-molecule force spectroscopy (SMFS) is a powerful tool to dissect molecular interactions that govern the stability and function of proteins. We applied SMFS to understand the effect of Zn2+ on the molecular interactions underlying the structure of rhodopsin. Force-distance curves obtained from SMFS assays revealed the strength and location of molecular interactions that stabilize structural segments within this receptor. The inclusion of ZnCl2 in SMFS assay buffer increased the stability of most structural segments. This effect was not mimicked by CaCl2, CdCl2, or CoCl2. Thus, Zn2+ stabilizes the structure of rhodopsin in a specific manner. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
