不同生长底物对培养背根神经节细胞的影响（简报）

发育期细胞和细胞外基质(extracellular matrix，ECM)之间的相互作用调节着细胞的功能，包括细胞的迁移、细胞骨架的构建、细胞的增值和分化。神经元“移居”体外后，失去了在体内所依托的组织学关系，必须黏附于一个固相表面才能生存，所以神经元只有在包被基质的培养器皿上才能存活，

Influence of substrates coated on cultured dorsal root ganglion cells]

Dorsal root ganglia from rat embryos were digested with trypsin to form single cell suspension. The cells were seeded on culture plates coated respectingly with poly-L-lysin (PLL), laminin (LN), PLL combined with LN, or collagen type I (CoI), then cultured in NB1 media. The cell survival and neurite outgrowth of cultured dorsal root ganglion cells were observed by phase-contrast microscopy. The results showed that the dorsal root ganglion neurons grown on PLL combined with LN were dispersively distributed at high survival rate, and those cells grown on CoI were clustered with thicker and longer neurites. It is suggested that the cell growth pattern could be influenced by different substances coated on the plates, and PLL combined with LN may provide a better substrum to culture and study single neuron soma and neurite.