| 二核苷酸重复多态性的非同位素检测及其在基因诊断中的应用 |
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| 引用本文： | 黄晓明 胡向阳. 二核苷酸重复多态性的非同位素检测及其在基因诊断中的应用[J]. 遗传学报, 1995, 22(2): 81-85 |
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| 作者姓名： | 黄晓明 胡向阳 |
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| 作者单位： | 南京铁道医学院科研所医学分子生物学室 |
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| 摘 要： | 本文报道了一种检测二核苷酸重复多态性的简便的非同位素法，利用重复序列两侧的特异引物进行ＰＣＲ扩增，产生的等位片段在薄层变性聚丙烯酰胺凝胶电泳上分离，再用灵敏的银染法显色。该方法不需要标记ＰＣＲ产物，简便、快速，分辨率可达１ｂｐ，并可用多对引物同时进行多重ＰＣＲ分析。用此方法对ＤＭＤ家系成员ｄｙｓｔｒｏｐｈｉｎ基因的５＇－脑型外显子止游区和３＇－非翻译区的两个（ＣＡ）。位点进行了扩增片段长度多态性分
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| 关 键 词： | 二核苷酸 重复多态性 聚合酶链反应 基因诊断 |
Non-radioactive Detection of Dinucleotide Repeat Polymorphisms and Its Application to Genetic Diagnosis |
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| Huang Xiaoming, Hu Xiangyang, Zong Hui, Gao Yizhi. Non-radioactive Detection of Dinucleotide Repeat Polymorphisms and Its Application to Genetic Diagnosis[J]. Journal of Genetics and Genomics, 1995, 22(2): 81-85 |
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| Authors: | Huang Xiaoming Hu Xiangyang Zong Hui Gao Yizhi |
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| Abstract: | We have developed a rapid and non-radioactive procedure for detecting dinucleotide repeat polymorphisms.Specific primers flanking the repeats were used in thepolymerase chain reaction(PCR).The generated allelic fragments were resolved bythin-layer denaturing polyacrylamide gel electrophoresis and visualized by improvedsilver staining. This method is simple, fast and without labelling of PCR products.Resolution of 1 bp can be easily obtained. It can also be used in multiplex PCR analysis of(CA)n loci. This method will be a valuable tool for linkage analysis andDNA diagnosis as demonstrated by its successful application to the CA dinucieotiderepeat haplotying of a DMD family.Received October 29.1993 |
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| Keywords: | Dinucleotide repeat polymorphism Polymerase chain reaction Gene diagnosis |
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