| 几种芽胞杆菌溶血素BL基因及其溶血素的检测 |
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| 引用本文： | 王利国,王琦,齐俊生,付学池,梅汝鸿.几种芽胞杆菌溶血素BL基因及其溶血素的检测[J].微生物学杂志,2007,27(3):21-23. |
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| 作者姓名： | 王利国 王琦 齐俊生 付学池 梅汝鸿 |
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| 作者单位： | 中国农业大学,农学与生物技术学院,北京,100094 |
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| 摘 要： | 用PCR方法对几种芽胞杆菌溶血素BL基因进行了检测,结果表明7株蜡样芽胞杆菌含有溶血素BL基因hblA、hblC、hblD,其他枯草芽胞杆菌、多粘类芽胞杆菌、地衣芽胞杆菌检测到部分溶血素基因;通过血平板培养的方法检测结果表明只有含有溶血素全部基因的菌株才会产生溶血环,从而为筛选不产生溶血素的有益芽胞杆菌奠定一定基础。
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| 关 键 词： | 芽胞杆菌 基因 溶血素 血平板 |
| 文章编号： | 1005-7021(2007)03-0021-03 |
| 修稿时间： | 2006年11月20 |
Detection of Hemolysin BL Genes and Hemolysin in Several Bacillus Species |
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| WANG Li-guo,WANG Qi,QI Jun-sheng,FU Xue-chi,Mei ru-hong.Detection of Hemolysin BL Genes and Hemolysin in Several Bacillus Species[J].Journal of Microbiology,2007,27(3):21-23. |
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| Authors: | WANG Li-guo WANG Qi QI Jun-sheng FU Xue-chi Mei ru-hong |
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| Abstract: | Hemolysin BL genes were detected from several Bacillus species using PCR method. The results showed that seven Bacillus cereus contained hblA,hblC,and hblD,partial hemolysin BL genes were also detected from B.subtilis,B.licheniformis, and B.polymyxa.The results of detection through cultivation on blood agar plate suggested that only those strains containing complete hemolysin BL genes could produce hemolysis ring,it thus laid a certain foundation for screening beneficial and hemolysin-nonproductive Bacillus strains. |
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| Keywords: | PCR |
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