Thecamoeba cosmophorea n. sp. (Amoebozoa,Discosea, Thecamoebida) — An example of sibling species within the genus Thecamoeba |
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| Institution: | 1. Campus de Ciencias Biológicas y Agropecuarias, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Km 15.5 Carretera Mérida-X’matkuil, Apdo. Postal 4-116 Itzimná, CP 97100 Mérida, YucatánMexico;2. Laboratorio de Biología Celular, Depto. Biomedicina de Enfermedades Infecciosas y Parasitarias, Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de Yucatán, Avenida Itzáes #490 x 59 Centro, CP 97000 Mérida, Yucatán, Mexico;1. Department of Molecular Biology & Immunology and Institute for Cancer Research, Graduate School of Biomedical Sciences, University of North Texas Health Science Center, Fort Worth, TX 76107, USA;2. Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA;3. Infectious Disease Division, Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX 77555, USA;4. Department of Chemistry, Yale University, 225 Prospect Street, PO Box 208107, New Haven, CT 06520, USA;1. Area of Parasitology, Department of Microbiology, Preventive Medicine and Public Health, Faculty of Medicine, University of Zaragoza, C/ Domingo Miral s/n, Zaragoza, Spain;2. Metropolitan District of Granada''s Epidemiology and Programs Unit, C/ Gustavo Dore, no. 3, Bajo, Granada, Spain;3. Department of Microbiology, University Hospital Virgen de las Nieves, Granada, Spain;4. Department of Microbiology, Faculty of Medicine, University of Granada, Avenida de Madrid, s/n, Granada, Spain;1. CHLAREAS-12, Rue Du Maconnais, F-54500 Vandoeuvre-lès-Nancy, France;2. Institute of Tropical Medicine and Specific Prophylaxis, Centre for Pathophysiology, Infectiology, and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090 Vienna, Austria;3. Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Setor de Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil |
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| Abstract: | A new species Thecamoeba cosmophorea n. sp. (Amoebozoa, Discosea, Thecamoebida) was isolated from leaf litter collected in the surroundings of Saint-Petersburg (Russia). This species resembles T. quadrilineata in light-microscopic morphology, but has certain morphological differences and significantly differs in 18S rRNA gene sequence. We performed a direct comparison of this newly isolated species with the Thecamoeba strain isolated from leaf litter in East Siberia (Russia) and identified as T. quadrilineata both at the morphological and the molecular level. There is no type strain of T. quadrilineata, and the type material on this species is represented with the stained slide by F.C. Page that he designated as neotype in 1977. The sequence of the 18S rRNA gene of this species deposited in the GenBank belongs to the isolate identified as T. quadrilineata by Rolf Michel in 1998 and deposited as CCAP 1583/10 strain. Hence we cannot be entirely sure that morphological and molecular data on T. quadrilineata belong to the same amoebae species. The use of molecular data for reliable species differentiation is getting obligate even within the amoebae genus Thecamoeba, which until recently was believed to be among few genera of naked lobose amoebae allowing morphological identification of species. |
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| Keywords: | Amoebozoa Morphology Phylogeny Thecamoebida |
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