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First controlled progenies checked by isozymic markers in cultivated yams Dioscorea cayenensis-rotundata
Authors:J Zoundjihekpon  S Hamon  B Tio-Touré  P Hamon
Institution:(1) Université Nationale de Côte d'Ivoire, Faculté des Sciences et Techniques, Laboratoire de Génétique, Abidjan 22, 22 BP 582, Ivory Coast;(2) ORSTOM, LRGAPT-BP, 1 Montpellier cedex, France;(3) UNESCO, 1 rue Mollis, 75015 Paris, France;(4) Université Montpellier III, Laboratoire de Zoogéographie, BP 5043 (or CIRAD-CA, BIOTROP-BP 5035), 1 Montpellier cedex, France
Abstract:As tested progeny have never been obtained, breeding studies on African yams (Dioscorea cayenensisrotundata) are scarce. We report here the first progenies checked by isoenzyme markers. This was made possible by the choice of well-known genitors one male (cv Zrezrou) and three females (cvs lsquoSopérérsquo, lsquoDahomeyrsquo and lsquoC 20rsquo)] and special hybridization conditions. Six enzymatic systems esterase (EST), isocitrate dehydrogenase (ICD), malate dehydrogenase (MDH), 6-phosphogluconate dehydrogenase (6PGD), shikimate dehydrogenase (SDH), and phosphoglucoisomerase (PGI)] were used to check the progenies and detect outbreeding. Despite the small number of progeny, it was possible to provide information on the genetics of the isoenzymatic systems.
Keywords:Yams  Controlled progenies  Isozymes Genetic analysis
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