激动素在黄瓜子叶器官分化中的作用

采用在培养初期分阶段供应激动素 (KT)的方法 ,研究了其对黄瓜子叶器官分化的影响 ,并对全程供应和不供应KT下子叶中内源多胺和内源激素的变化进行了动态测定。结果表明 :1)供应KT使不定根分化率下降 ,不影响营养芽的分化 ,而对于花芽分化则是必需的。2 )供应KT使多胺中腐胺 (Put)含量变化最为显著 ,在培养第 2、 6d出现峰值 ,在第 4d呈低谷 ,对照则在第 4d出现峰值。 3)供应KT使培养 2d的子叶中吲哚乙酸 (IAA)和脱落酸(ABA)含量显著降低 ,4d时IAA含量显著升高。据上结果 ,就这些生理变化和花芽分化之间是否存在相关进行了充分讨论。

Effect of Kinetin on the Organ Differentiation in Cucumber Cotyledons

The effect of Kinetin (KT) on organ differentiation in cucumber cotyledons was studied with the provision of KT by stages at the beginning of culture. The results showed that: 1)Provision of KT caused a descending rooting, had almost no effect on vegetable bud differentiation, and was utterly essential to the flower bud formation. 2)Provision of KT caused the content of Putrescine (Put) to change greatly. It had two peak values, one occurred on the 2nd day, the other on the 6th day, but on the 4th day its content decreased sharply. 3)Provision of KT caused the contents of IAA and ABA to decrease significantly on the 2nd day and to rise sharply on the 4th day for IAA. Based on these results, we discuss the relationship between these physiological changes and flower differentiation.