Effects of dietary selenium and vitamin E concentrations on phospholipid hydroperoxide glutathione peroxidase expression in reproductive tissues of pubertal maturing male rats |
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Authors: | Xin Gen Lei Deborah A Ross John E Parks Gerald F Combs |
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Institution: | (1) Department of Animal Science and Division of Nutritional Sciences, Cornell University, 14853 Ithaca, NY |
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Abstract: | Phospholipid hydroperoxide glutathione peroxidase (PHGPX) is the second intracellular selenium (Se)-dependent glutathione
peroxidase (GSH-Px) identified in mammals. Our objectives were to determine the effect of dietary vitamin E and Se levels
on PHGPX activity expression in testis, epididymis, and seminal vesicles of pubertal maturing rats, and the relationship of
PHGPX expression with testicular development and sperm quality. Forty Sprague-Dawley male weanling rats (21-d old), were initially
fed for 3 wk a torula yeast basal diet (containing 0.05 mg Se/kg) supplemented with marginal levels of Se (0.1 mg/kg as Na2SeO3) and vitamin E (25 IU/kg as all-rac-α-tocopheryl acetate). Then, rats were fed the basal diets supplemented with 0 or 0.2
mg Se/kg and 0 or 100 IU vitamin E/kg diet during the 3-wk period of pubertal maturing. Compared with the Se-supplemented
rats, those fed the Se-deficient diets retained 31, 88, 67, and 50% of Se-dependent GSH-Px activities in liver, testis, epididymis,
and seminal vesicles, respectively. Testes and seminal vesicles had substantially higher (5-to 20-fold) PHGPX activity than
liver. Dietary Se deficiency did not affect PHGPX activities in the reproductive tissues, but reduced PHGPX activity in liver
by 28% (P < 0.0001). Dietary vitamin E supplementation did not affect PHGPX activity in liver, whereas it raised PHGPX activity
in seminal vesicles by 43% (P < 0.005). Neither dietary vitamin E nor Se levels affected body weight gains, reproductive organ
weights, or sperm counts and morphology. In conclusion, expression of PHGPX activity in testis and seminal vesicles was high
and regulated by dietary Se and vitamin E differently from that in liver. |
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Keywords: | Selenium vitamin E glutathione peroxidase rat testicular tissues phospholipid hydroperoxide reproduction |
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