The localization of calcium by X-ray microanalysis in myopathic muscle fibers |
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Authors: | Ernö Dux Ida Tóth László Dux Ferenc Joó |
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Institution: | (1) Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701 Szeged, Hungary;(2) Institute of Biochemistry, Medical University of Szeged, H-6720 Szeged, Hungary |
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Abstract: | Summary An electron histochemical study was undertaken to localize calcium with ammonium oxalate precipitation technique in soleus muscle of rat in normal cases and in myopathy induced experimentally by a prolonged treatment of 2,4-dichlorophenoxyacetate (2,4-D). The calcium content of precipitates was detected by energy-dispersive X-ray microanalysis. In normal cases, the electron dense precipitates containing calcium were mainly found in the vesicles of sarcoplasmic reticulum, whereas in 2,4-D induced myopathy the deposits were shifted near the Z line into the myofibrils. Calcium, because the uptake into sarcoplasmic vesicles was inhibited by 2,4-D, could attach to other binding sites, such as to the troponin-C.A long-lasting binding of calcium might lead to a prolonged activation of the actin-myosin system.Fellow of the Max-Planck Society at the Abteilung Neurobiologie, Neuroanatomie, Max-Planck-Institut für biophysikalische Chemie, Göttingen. Federal Republic of Germany |
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