Establishment of the comet assay in the freshwater snail Biomphalaria glabrata (Say, 1818) |
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| Authors: | Grazeffe Vanessa Siqueira Tallarico Lenita de Freitas Pinheiro Alessandro de Sá Kawano Toshie Suzuki Miriam Fussae Okazaki Kayo Pereira Carlos Alberto de Bragança Nakano Eliana |
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| Institution: | Laboratório de Parasitologia/Malacologia, Instituto Butantan, Avenida Vital Brasil, 1500, CEP 05503-900, S?o Paulo, Brazil. |
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| Abstract: | The single cell gel electrophoresis or the comet assay was established in the freshwater snail Biomphalaria glabrata. For detecting DNA damage in circulating hemocytes, adult snails were irradiated with single doses of 2.5, 5, 10 and 20 Gy of (60)Co gamma radiation. Genotoxic effect of ionizing radiation was detected at all doses as a dose-related increase in DNA migration. Comet assay in B. glabrata demonstrated to be a simple, fast and reliable tool in the evaluation of genotoxic effects of environmental mutagens. |
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