长期喷施ABA对云杉幼苗生长和生理特性的影响

采用单因素盆栽实验，通过叶面喷施5、10、15和20mg·L^-1 4个浓度的ABA溶液，研究了长期外源ABA处理对云杉（Piceaasperata）幼苗生长及生理特性的影响。5年的研究结果表明：长期不同浓度ABA处理显著影响了云杉幼苗的多种生长及生理生化指标。当ABA浓度为5、10和15mg·L^-1叫时有利于云杉幼苗根重、茎重和总生物量的积累，并且提高了叶片中可溶性蛋白和脯氨酸的含量，降低了MDA含量：20mg·L^-1 ABA处理使幼苗的叶重、总生物量、脯氨酸及可溶性糖含量显著下降，明显增加了叶片中MDA含量。此外，各浓度ABA处理均显著降低了云杉幼苗的株高、叶绿素含量以及SOD和APX活性。本研究结果显示，长期ABA处理对云杉幼苗生长和生理特性的影响与所喷施的ABA浓度有关，长期高浓度ABA（20mg·L^-1）处理不利于云杉幼苗生长。

Growth and Physiological Responses of Picea asperata Seedlings to Long-term Abscisic Acid Application

The paper describes the effects of long-term application of abscisic acid （ABA） at different concentrations in Picea asperata at the Maoxian Ecological Experimental Station of Chinese Academy of Sciences during a 5-year pot experiment. Longterm exogenous ABA application significantly affected the height and biomass （root, stem, and leaf weight）; chlorophyll, osmoprotectant （proline, soluble sugar, soluble protein）, and malonic dialdehyde （MDA） content; and antioxidative enzyme activity. ABA treatments at 5 mg·L^-1, 10mg·L^-1 and 15mg·L^-1 significantly improved root weight, stem weight and total biomass; increased the soluble protein and proline content; and reduced MDA content in needles. However, the highest application of ABA （20 mg·L^-1） caused a significant reduction in accumulation of chlorophyll, free proline and soluble sugar and a significant increase in MDA content in needles. These results suggest that the effects of exogenous ABA application on Picea asperata is concentration dependent, and long-term high exogenous ABA （20 mg·L^-1） is unfavorable for plant growth. Moreover, the height, chlorophyll content, and activities of superoxide dismutase and ascorbate peroxidase were significantly reduced with application of exogenous ABA.