| 应用纯化的重组Epstein-Barr病毒早期抗原建立检测鼻咽癌病人血清IgA/EA抗体的ELISA方法 |
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| 引用本文： | 金传芳,WolfH.,曾毅.应用纯化的重组Epstein-Barr病毒早期抗原建立检测鼻咽癌病人血清IgA/EA抗体的ELISA方法[J].病毒学报,1990(3). |
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| 作者姓名： | 金传芳 WolfH. 曾毅 |
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| 作者单位： | 中国预防医学科学院病毒学研究所，西德慕尼黑Pettenkofer研究所，中国预防医学科学院病毒学研究所 北京，北京 |
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| 摘 要： | 利用凝胶和离子交换柱(Mono Q)两次层析,将大肠杆菌表达的EB病毒早期抗原P138片段多肽纯化。以此P138为抗原,增加鼠抗人IgA单克隆抗体以扩大IgA的反应,建立了三步ELISA法。用本法检查了100例鼻咽癌病人和63例正常人血清中抗EB病毒IgA/EA抗体,病人血清的阳性检出率为86％,正常人有3例阳性(4.7％)。此结果表明,三步ELISA法较常用的间接ELISA法(阳性检出率为71％)敏感。
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| 关 键 词： | 重组EB病毒早期抗原 鼻咽癌 酶联免疫吸附试验 |
PURIFICATION OF RECOMBINANT EPSTEINBARR VIRUS EARLY ANTIGEN AND APPLICATING TO DETECT IgA IN SERA FROM PATIENTS WITH NASOPHARYNGEAL CARCINOMA BY ELISA |
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| Jin Chuanfang H. Wolf Zeng Yi.PURIFICATION OF RECOMBINANT EPSTEINBARR VIRUS EARLY ANTIGEN AND APPLICATING TO DETECT IgA IN SERA FROM PATIENTS WITH NASOPHARYNGEAL CARCINOMA BY ELISA[J].Chinese Journal of Virology,1990(3). |
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| Authors: | Jin Chuanfang H Wolf Zeng Yi |
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| Abstract: | We have purified recombinant Epstein-Barr virus ( EBV ) early antigen protein 138-kD by gel chromatography and ion-exchange chromatography. A sensitive three-step ELISA, based on the use of purified recombinant early antigen Pl38, was developed to detect IgA antibody, in which a monoclo- nal antibody directed against human IgA antibody was adopted for amplifying the reaction. Examination of IgA/EA-P138 antibody in sera from 100 patients with nasopharyngeal carcinoma ( NPC ) shoved a positive percentage of 86% ,while only 4.7% were positive in 63 healthy individuals. The positive rates of these two sample groups tested by indirect ELISA are 71% and 0% ,respectively. It seems that three step ELISA is more sensitive than indirect ELISA. |
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| Keywords: | Recombinant Epstein-Barr virus early antigen Nasopharyngeal Carcinoma ELISA |
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