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The human glucagon receptor encoding gene: structure, cDNA sequence and chromosomal localization |
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| Authors: | Si Lok Joseph L. Kuijper Laura J. Jelinek Janet M. Kramer Theodore E. Whitmore Cindy A. Sprecher Shannon Mathewes Francis J. Grant Shaula H. Biggs Gary B. Rosenberg Paul O. Sheppard Patrick J. O''Hara Donald C. Foster Wayne Kindsvogel |
| Affiliation: | a Departments of Molecular and Cellular Biology: ZymoGenetics Inc., Seattle, WA 95105, USA b Departments of Molecular Pharmacology: ZymoGenetics Inc., Seattle, WA 95105, USA c Departments of ONA Chemistry and Computer Science: ZymoGenetics Inc., Seattle, WA 95105, USA d Departments of Protein Chemistry, ZymoGenetics Inc., Seattle, WA 95105, USA |
| Abstract: | Characterization of the human glucagon-receptor-encoding gene (GGR) should provide a greater understanding of blood glucose regulation and may reveal a genetic basis for the pathogenesis of diabetes. A cDNA encoding a complete functional human glucagon receptor (GGR) was isolated from a liver cDNA library by a combination of polymerase chain reaction and colony hybridization. The cDNA encodes a receptor protein with 80% identity to rat GGR that binds [125I] glucagon and transduces a signal leading to increases in the concentration of intracellular cyclic adenosine 3′,5′-monophosphate. Southern blot analysis of human DNA reveals a hybridization pattern consistent with a single GGR locus. In situ hybridization to metaphase chromosome preparations maps the GGR locus to chromosome 17q25. Analysis of the genomic sequence shows that the coding region spans over 5.5 kb and is interrupted by 12 introns. |
| Keywords: | G-protein-coupled receptor sequence alignment signal transduction recombinant DNA transmembrane domain introns |
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