Temperature-Regulated Surface Plasmon Resonance Imaging System for Bioaffinity Sensing |
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| Authors: | Pengfei Zhang Le Liu Yonghong He Yanhong Ji Jun Guo Hui Ma |
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| Institution: | 1.Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of optical imaging and sensing, Graduate School at Shenzhen,Tsinghua University,Shenzhen,China;2.Department of Physics,Tsinghua University,Beijing,China;3.Institute of Green Chemistry and Energy, Graduate School at Shenzhen,Tsinghua University,Shenzhen,China;4.MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science,South China Normal University,Guangzhou,China |
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| Abstract: | We describe a temperature-regulated surface plasmon resonance (SPR) imaging biosensor in this article. The sample temperature can be regulated for specific requirements of the bioaffinity sensing, and stabilized to suppress the measurement noise caused by temperature fluctuations. The water thermo optic coefficient is measured to test the temperature regulation performance. The protein interaction is monitored to demonstrate the feasibility of this system for real-time biomolecular interaction analysis. This temperature-regulated SPR imaging biosensor can be readily implemented by adding the common water path and peristaltic pump to the conventional SPR imaging system, which may provide an economical and convenient scheme to improve the analysis accuracy and quality of bioaffinity sensing using SPR sensing platform. |
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