Determination of serum protein concentration in nanoliter blood samples using fluorescamine or 9-phthalaldehyde. |
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| Authors: | J W Viets W M Deen J L Troy B M Brenner |
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| Institution: | 1. Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA;2. Laboratory of Kidney and Electrolyte Physiology, Peter Bent Brigham Hospital, Boston, Massachusetts 02115 USA |
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| Abstract: | Fluorometric procedures were developed to permit measurement of total protein concentration in nanoliter serum samples, using either fluorescamine or o-phthalaldehyde. The sensitivities of assays using these two reagents were similar, but the o-phthalaldehyde method was found to be somewhat simpler and more reproducible. Accurate measurements could be obtained on serum samples of 4 to 5 nl with either reagent, by using a serum standard. Fluorescence differed considerably among individual serum proteins, albumin generally showing greater fluorescence than globulins. Small molecular weight species in serum did not contribute appreciably to total serum fluorescence with either reagent. |
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