Comparative assessment of the intracellular survival of the Burkholderia pseudomallei bopC mutant |
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Authors: | Varintip Srinon Sunsiree Muangman Nithima Imyaem Veerachat Muangsombut Natalie R. Lazar Adler Edouard E. Galyov Sunee Korbsrisate |
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Affiliation: | 1. Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand 2. Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, LE1 9HN, UK
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Abstract: | Burkholderia pseudomallei, the causative agent of melioidosis, is a Gram-negative saprophytic bacterium capable of surviving within phagocytic cells. To assess the role of BopC (a type III secreted effector protein) in the pathogenesis of B. pseudomallei, a B. pseudomallei bopC mutant was used to infect J774A.1 macrophage-like cells. The bopC mutant showed significantly reduced intracellular survival in infected macrophages compared to wild-type B. pseudomallei. In addition, the bopC mutant displayed delayed escape from endocytic vesicles compared with the wild-type strain. This indicates that BopC is important, and at least in part, needed for intracellular survival of B. pseudomallei. |
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