| 小鼠HMGB1启动子荧光素酶报告基因的构建及功能鉴定 |
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| 引用本文： | 欧阳华伟,罗成群,周建大,卜平元.小鼠HMGB1启动子荧光素酶报告基因的构建及功能鉴定[J].生命科学研究,2010,14(2):137-140. |
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| 作者姓名： | 欧阳华伟 罗成群 周建大 卜平元 |
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| 作者单位： | 中南大学湘雅三医院烧伤整形外科,中国湖南,长沙,410013 |
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| 摘 要： | 利用PCR技术扩增小鼠高迁移率族蛋白1(HMGB1)基因启动子序列,构建小鼠HMGB1启动子荧光素酶报告基因pGL3-basic-HMGB1.经PCR、酶切及测序鉴定后,用脂质体法将pGL3-basic-HMGB1转入巨噬细胞264.7中,并应用萤光素酶测定系统检测其活性.检测结果显示pGL3-basic-HMGB1具有启动子活性.小鼠HMGB1启动子荧光素酶报告基因pGL3-basic-HMGB1的成功构建,为进一步研究HMGB1提供基本材料.
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| 关 键 词： | 高迁移率族蛋白1 启动子 荧光素酶 报告基因 |
Construction and Identification of Luciferase Reporter Gene Containing Mouse HMGB1 Promoter |
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| OUYANG Hua-wei,LU Cheng-qun,ZHOU Jian-da,PU Ping-yuan.Construction and Identification of Luciferase Reporter Gene Containing Mouse HMGB1 Promoter[J].Life Science Research,2010,14(2):137-140. |
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| Authors: | OUYANG Hua-wei LU Cheng-qun ZHOU Jian-da PU Ping-yuan |
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| Institution: | OUYANG Hua-wei,LUO Cheng-qun,ZHOU Jian-da,PU Ping-yuan(Department of Burns , Plastic Surgery,Third Xiangya Hospital,Central South University,Changsha 410013,Hunan,China) |
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| Abstract: | In order to construct the luciferase reporter gene containing mouse HMGB1 promoter pGL3-basicHMGB1,the promoter of high mobility group box 1(HMGB1)gene was amplified from mouse genomic DNA by Polymerase Chain Reaction(PCR).The recombinant PGL3-basic-HMGB1 was transfected into 264.7 cells by lipofectamine after it was confirmed by PCR analysis,restriction enzyme digestion,and DNA sequencing,then the activity of luciferase was detected.The result demonstrated that the PGL3-basicHMGB1 had the promoter activity... |
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| Keywords: | HMGB1 promoter luciferase reporter genes |
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