MDR-like ABC transporter AtPGP4 is involved in auxin-mediated lateral root and root hair development |
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| Authors: | Santelia Diana Vincenzetti Vincent Azzarello Elisa Bovet Lucien Fukao Yoichiro Düchtig Petra Mancuso Stefano Martinoia Enrico Geisler Markus |
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| Affiliation: | Zurich-Basel Plant Science Center, Institute of Plant Biology, Molecular Plant Physiology, University of Zürich, Switzerland. |
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| Abstract: | Previous data have suggested an involvement of MDR/PGP-like ABC transporters in transport of the plant hormone auxin and, recently, AtPGP1 has been demonstrated to catalyze the primary active export of auxin. Here we show that related isoform AtPGP4 is expressed predominantly during early root development. AtPGP4 loss-of-function plants reveal enhanced lateral root initiation and root hair lengths both known to be under the control of auxin. Further, atpgp4 plants show altered sensitivities toward auxin and the auxin transport inhibitor, NPA. Finally, mutant roots reveal elevated free auxin levels and reduced auxin transport capacities. These results together with yeast growth assays suggest a direct involvement of AtPGP4 in auxin transport processes controlling lateral root and root hair development. |
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| Keywords: | PAT, polar auxin transport IAA, indole-3-acetic acid NPA, 1-N-naphtylphtalamic acid LR, lateral root RH, root hair ACC, aminocyclopropane carboxylic acid AVG, ethylene biosynthesis inhibitor aminovinylglycine GC-MS, gas chromatography-coupled mass spectrometry MS, Murashige and Skoog basal medium SE, standard error ORF, open reading frame |
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